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KMID : 1025520040460010077
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Journal of Animal Science and Technology
2004 Volume.46 No. 1 p.77 ~ p.82
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Purification of Angiogenin from Bovine Milk
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Abstract
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This study was carried out to establish the purification protocol of angiogenin(ANG) from bovine milk. The purification of ANG from bovine milk was performed by using cation chromatography, high-performance liquid chromatography and gel-filtration. We obtained the ANG protein have the molecular weight of about 14 kD by SDS-PAGE analysis. This protein was confirmed as ANG by NH₂-terminal sequence analysis of the first 15 amino acids. Identified amino acids revealed the protein to be identical to that previously reported for bovine ANG.
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